abstract：:Polymorphisms in mitochondrial DNA (mtDNA) were analyzed in 152 samples from the Polish population using restriction enzymes AvaI, BamHI, HaeII, HpaI and PstI. Additionally, each sample was classified into the appropriate haplogroup. When required, appropriate fragments were sequenced to establish the exact polymorphi...

journal_title：Acta biochimica Polonica

authors： Piechota J,Tońska K,Nowak M,Kabzińska D,Lorenc A,Bartnik E

更新日期：2004-01-01 00:00:00

abstract：:Nowadays, scientists may learn a lot about the organisms studied just by analyzing their genetic material. This requires the development of methods of reading genomes with high accuracy. It has become clear that the knowledge of the changes occurring within a viral genome is indispensable for effective fighting of the...

journal_title：Acta biochimica Polonica

authors： Błazewicz J,Figlerowicz M,Formanowicz P,Kasprzak M,Nowierski B,Styszyński R,Szajkowski L,Widera P,Wiktorczyk M

更新日期：2004-01-01 00:00:00

abstract：:The capabilities of porcine adrenal cortex mitochondria to oxidize glycerol-3-phosphate (GP) were studied. In comparison with bovine adrenal cortex mitochondria, porcine mitochondria oxidized GP about three times more actively (18.9 vs 6.1 nmol O(2)/min per mg protein in the presence of ADP) and the activity of mitoch...

journal_title：Acta biochimica Polonica

authors： Popinigis J,Antosiewicz J,Kaczor JJ,Rauchová H,Lenaz G

更新日期：2004-01-01 00:00:00

abstract：:Spontaneous mutations arise not only in exponentially growing bacteria but also in non-dividing or slowly dividing stationary-phase cells. In the latter case mutations are called adaptive or stationary-phase mutations. High spontaneous mutability has been observed in temperature sensitive Escherichia coli dnaQ49 strai...

journal_title：Acta biochimica Polonica

authors： Nowosielska A,Nieminuszczy J,Grzesiuk E

更新日期：2004-01-01 00:00:00

abstract：:Restricting food intake to a level below that consumed voluntarily (85%, 70% and 50% of the ad libitum energy intake for 3 or 30 days) and re-feeding ad libitum for 48 h results in an increase of malic enzyme (ME) gene expression in rat white adipose tissue. The increase of ME gene expression was much more pronounced ...

journal_title：Acta biochimica Polonica

authors： Stelmanska E,Korczynska J,Swierczynski J

更新日期：2004-01-01 00:00:00

abstract：:Signalling concerns the transfer of information from one body, a source, to another, a receiver in order to stimulate activity. The problem arises with the word information. It is defined as what is transferred in a sequence of things, say between people, e.g. words or signs. The idea of signalling between people is t...

journal_title：Acta biochimica Polonica

authors： Williams RJ

更新日期：2004-01-01 00:00:00

abstract：:beta-Secretase, a beta-site amyloid precursor protein (APP) cleaving enzyme (BACE), participates in the secretion of beta-amyloid peptides (Abeta), the major components of the toxic amyloid plaques found in the brains of patients with Alzheimer's disease (AD). According to the amyloid hypothesis, accumulation of Abeta...

journal_title：Acta biochimica Polonica

authors： Nawrot B

更新日期：2004-01-01 00:00:00

abstract：:Dansylated analogues of the potent and selective micro opioid peptide agonist [Dmt(1)]DALDA (H-Dmt-D-Arg-Phe-Lys-NH(2); Dmt = 2',6'-dimethyltyrosine) were prepared either by substitution of N(beta)-dansyl-alpha,beta-diaminopropionic acid or N(epsilon)-dansyllysine for Lys(4), or by attachment of a dansyl group to the ...

journal_title：Acta biochimica Polonica

authors： Berezowska I,Lemieux C,Chung NN,Zelent B,Schiller PW

更新日期：2004-01-01 00:00:00

abstract：:Cytochrome c is an important electron transfer protein in the respiratory chain, shuttling electrons from cytochrome c reductase to cytochrome c oxidase. Extensive chemical modification studies indicate significant electrostatic interactions between these proteins and show that all structural and conformational change...

journal_title：Acta biochimica Polonica

authors： Trynda-Lemiesz L

更新日期：2004-01-01 00:00:00

abstract：:The systemic inflammatory reaction (acute phase response) is induced by many noxious stimuli but in all cases the inflammatory cytokines, such as interleukin-1-beta (IL-1beta) and interleukin-6 (IL-6), are involved. Liver cell response to inflammation manifested by a characteristic change in the profile of synthesized...

journal_title：Acta biochimica Polonica

authors： Koj A,Jura J

更新日期：2003-01-01 00:00:00

abstract：:Regulation of proteolytic enzyme activity is an essential requirement for cells and tissues because proteolysis at a wrong time and location may be lethal. Proteases are synthesized as inactive or less active precursor molecules in order to prevent such inappropriate proteolysis. They are activated by limited intra- o...

journal_title：Acta biochimica Polonica

authors： Wiederanders B

更新日期：2003-01-01 00:00:00

abstract：:Ammonium salt of N-(dithiocarboxy)sarcosine (DTCS) chelated to ferrous salt was tested as an NO-metric spin trap at room temperature for ex vivo measurement of (.)NO production in murine endotoxaemia. In a chemically defined in vitro model system EPR triplet signals of NO-Fe(DTCS)(2) were observed for as long as 3 hou...

journal_title：Acta biochimica Polonica

authors： Plonka PM,Wisniewska M,Chlopicki S,Elas M,Rosen GM

更新日期：2003-01-01 00:00:00

abstract：:We investigated the effect of the cyclic AMP-protein kinase A (PKA) signalling pathway on renal Na(+),K(+)-ATPase and ouabain-sensitive H(+),K(+)-ATPase. Male Wistar rats were anaesthetized and catheter was inserted through the femoral artery into the abdominal aorta proximally to the renal arteries for infusion of th...

journal_title：Acta biochimica Polonica

authors： Bełtowski J,Marciniak A,Wójcicka G,Górny D

更新日期：2003-01-01 00:00:00

abstract：:Progressive decline in fertility and sperm quality has been reported over the last few decades, especially in industrialized nations. It has been proposed that exposure to factors that induce damage in DNA of spermatogenic cells may significantly contribute to impaired fertility. Here, the (32)P-postlabelling method w...

journal_title：Acta biochimica Polonica

authors： Horak S,Polańska J,Widłak P

更新日期：2003-01-01 00:00:00

abstract：:Fibrin split product D-dimer (DD) is most probably involved in the development of vascular disorders. At 1.5 microM concentration DD inhibited the incorporation of D-[1-(3)H]glucosamine hydrochloride and [2-(14)C]acetate x Na into pericellular heparan sulphate (HS) of rabbit coronary endothelial cells without affectin...

journal_title：Acta biochimica Polonica

authors： Yevdokimova NY,Veselovska LD,Gogolinska GK,Buchanevich OM,Kosyakova GV,Gritsenko PG,Lugovskoj EV,Komisarenko SV

更新日期：2003-01-01 00:00:00

abstract：:The use of nucleotides and their analogs in the pharmacological studies of nucleotide receptors (P2 class) should be preceded by detailed studies on their degradation connected with ecto-enzymes of a given cell type. In the present studies we have analyzed stability of some phosphorothioate and phosphonate analogs of ...

journal_title：Acta biochimica Polonica

authors： Gendaszewska-Darmach E,Maszewska M,Zakłos M,Koziołkiewicz M

更新日期：2003-01-01 00:00:00

abstract：:This brief resume enumerates the multiple actions of melatonin as an antioxidant. This indoleamine is produced in the vertebrate pineal gland, the retina and possibly some other organs. Additionally, however, it is found in invertebrates, bacteria, unicellular organisms as well as in plants, all of which do not have a...

journal_title：Acta biochimica Polonica

authors： Reiter RJ,Tan DX,Mayo JC,Sainz RM,Leon J,Czarnocki Z

更新日期：2003-01-01 00:00:00

abstract：:Nematodes were found to synthesize phosphorylcholine-containing molecules not present in higher organisms, i.e. phosphorylcholine-substituted glycosphingolipids and (glyco)proteins. Investigations on the biosynthesis of these structures provided first biochemical evidence for the presence of the Kennedy and Bremer-Gre...

journal_title：Acta biochimica Polonica

authors： Lochnit G,Geyer R

更新日期：2003-01-01 00:00:00

abstract：:Description of the recognition specificity between proteins and nucleic acids at the level of molecular interactions is one of the most challenging tasks in biophysics. It is key to understanding the course and control of gene expression and to the application of the thus acquired knowledge in chemotherapy. This revie...

journal_title：Acta biochimica Polonica

authors： Stolarski R

更新日期：2003-01-01 00:00:00

abstract：:Endogenous peptide antibiotics are known as evolutionarily old components of innate immunity. Due to interaction with cell membrane these peptides cause permeabilization of the membrane and lysis of invading microbes. However, some studies proved that antimicrobial peptides are universal multifunctional molecules and ...

journal_title：Acta biochimica Polonica

authors： Kamysz W,Okrój M,Łukasiak J

更新日期：2003-01-01 00:00:00

abstract：:We assessed a relationship between the level of 8-oxodG in leukocyte DNA measured with the high performance liquid chromatography with electrochemical detection (HPLC/EC) technique and urinary excretion of the modified nucleoside/base analysed with a recently developed methodology involving HPLC prepurification follow...

journal_title：Acta biochimica Polonica

authors： Foksinski M,Gackowski D,Rozalski R,Olinski R

更新日期：2003-01-01 00:00:00

abstract：:This article reviews the evidence for the danger model in the context of immune response to tumors and the insufficiency of the immune system to eliminate tumor growth. Despite their potential immunogenicity tumors do not induce significant immune responses which could destroy malignant cells. According to the danger ...

journal_title：Acta biochimica Polonica

authors： Kowalczyk DW

更新日期：2002-01-01 00:00:00

abstract：:Spectroscopic, photochemical and biological properties of indocyanine green (ICG) are presented. Light over 800 nm is effectively absorbed by ICG. This property as well as photochemical behaviour of ICG make it a very suitable dye for photodynamic treatment of melanoma cells. Cytotoxicity of ICG itself and the effect ...

journal_title：Acta biochimica Polonica

authors： Urbanska K,Romanowska-Dixon B,Matuszak Z,Oszajca J,Nowak-Sliwinska P,Stochel G

更新日期：2002-01-01 00:00:00

abstract：:Glycophorin A (GPA), the major sialoglycoprotein of the human erythrocyte membrane, was isolated from erythrocytes of healthy individuals of blood groups A, B and O using phenol-water extraction of erythrocyte membranes. Interaction of individual GPA samples with three lectins (Psathyrella velutina lectin, PVL; Tritic...

journal_title：Acta biochimica Polonica

authors： Krotkiewska B,Pasek M,Krotkiewski H

更新日期：2002-01-01 00:00:00

abstract：:We here report the influence of the cell cycle abrogator UCN-01 on RKO human colon carcinoma cells differing in p53 status following exposure to two DNA damaging agents, the topoisomerase inhibitors etoposide and camptothecin. Cells were treated with the two drugs at the IC90 concentration for 24 h followed by post-in...

journal_title：Acta biochimica Polonica

authors： Bozko P,Larsen AK,Raymond E,Skladanowski A

更新日期：2002-01-01 00:00:00

abstract：:Recently we observed that the transcription factors Sp1 and Sp3 bind to the CTCCTCCTC sequence located between positions -194 and -172 of the alphaV promoter region and are directly involved in the regulation of transcriptional activity of the alphaV gene in human umbilical vascular endothelial cells (HUVECs) (Czyz & ...

journal_title：Acta biochimica Polonica

authors： Czyz M,Stasiak M,Boncela J,Cierniewsk CS

更新日期：2002-01-01 00:00:00

abstract：:Patients with colorectal carcinoma showed statistically significant lower values of transferrin saturation, total iron binding capacity and serum iron level as compared with control group, while the level of ferritin and the size of labile iron pool in carcinoma patients were higher, although this difference was not s...

journal_title：Acta biochimica Polonica

authors： Gackowski D,Kruszewsk M,Banaszkiewicz Z,Jawien A,Olinski R

更新日期：2002-01-01 00:00:00

abstract：:Two recently synthesized groups of acridine cytostatics containing fused heterocyclic ring(s): pyrazoloacridines (PAC) and pyrazolopyrimidoacridines (PPAC) were tested in regard to their in vitro cytotoxic activity towards a panel of sensitive and resistant human tumor cell lines. The obtained results corroborate our ...

journal_title：Acta biochimica Polonica

authors： Bontemps-Gracz MM,Kupiec A,Antonini I,Borowski E

更新日期：2002-01-01 00:00:00

abstract：:RNA nucleoside triphosphatases (NTPase)/helicases represent a large family of proteins that are ubiquitously distributed over a wide range of organisms. The enzymes play essential role in cell development and differentiation, and some of them are involved in transcription and replication of viral single-stranded RNA g...

journal_title：Acta biochimica Polonica

authors： Borowski P,Niebuhr A,Schmitz H,Hosmane RS,Bretner M,Siwecka MA,Kulikowski T

更新日期：2002-01-01 00:00:00

abstract：:A new approach has been developed to study binding of a ligand to a macromolecule based on the diffusion process. In terms of the Fick's first law, the concentration of free ligand in the presence of a protein can be determined by the measurement of those ligands which are diffused out. This method is applied to the s...

journal_title：Acta biochimica Polonica

authors： Housaindokht MR,Bahrololoom M,Tarighatpoor S,Mossavi-Movahedi AA

更新日期：2002-01-01 00:00:00

abstract：:Computer modelling related to the real dimensions of both the whole filament and the myosin molecule subfragments has revealed two alternative modes for myosin molecule packing which lead to the head disposition similar to that observed by EM on the surface of the cross-bridge zone of the relaxed vertebrate skeletal m...

journal_title：Acta biochimica Polonica

authors： Skubiszak L,Kowalczyk L

更新日期：2002-01-01 00:00:00

abstract：:A cytosolic acetyl-CoA hydrolase (CACH) cDNA has been isolated from mouse liver cDNA library and sequenced. Recombinant expression of the cDNA in insect cells resulted in overproduction of active acetyl-CoA hydrolyzing enzyme protein. The mouse CACH cDNA encoded a 556-amino-acid sequence that was 93.5% identical to ra...

journal_title：Acta biochimica Polonica

authors： Suematsu N,Okamoto K,Isohashi F

更新日期：2002-01-01 00:00:00

abstract：:So far, azide has been consistently reported to act as an inhibitor of metal enzymes, especially copper proteins. The present work shows that azide can also act as an activator of polyphenol oxidase II (PPO II) from tobacco leaves. From the square-wave voltammetry of native PPO II, peroxide-PPO II complex and azide-PP...

journal_title：Acta biochimica Polonica

authors： Shi C,Liu Q,Dai Y,Xie Y,Xu X

更新日期：2002-01-01 00:00:00

abstract：:An approximately threefold increase in glycogenolytic activity of the neutral alpha-1,4-glucosidase and a twofold increase in the same activity of the acid isoform have been found in extracts of anaplastic astrocytoma and glioblastoma multiforme tumors of brain tissue. "Maltase activity" of the respective enzymes incr...

journal_title：Acta biochimica Polonica

authors： Kotoński B,Wilczek J,Madej J,Zarzycki A,Hutny J

更新日期：2001-01-01 00:00:00

abstract：:Recently Boratyński & Roy (Glycoconjugate J., 1998, 15, 131) described a fast and convenient procedure for the synthesis of glycoconjugates. In the present study we used ESI-MS and circular dichroism as tools to analyze non-enzymatic glycation products of proteins and peptides. We discuss influence of reaction conditi...

journal_title：Acta biochimica Polonica

authors： Stefanowicz P,Boratyński J,Kańska U,Petry I,Szewczuk Z

更新日期：2001-01-01 00:00:00

abstract：:Temporin A (TA) and a cecropin A-temporin A hybrid peptide (CATA) were synthesized and assayed for their hemolytic, anticoagulant, and antifungal properties. CATA retains significant antifungal activity, is less hemolytic than TA, and inhibits blood coagulation. These results recommend further studies of the biologica...

journal_title：Acta biochimica Polonica

authors： Wade D,Silveira A,Rollins-Smith L,Bergman T,Silberring J,Lankinen H

更新日期：2001-01-01 00:00:00

abstract：:Asparaginases catalyze the hydrolysis of asparagine to aspartic acid and ammonia. Enzymes with asparaginase activity play an important role both in the metabolism of all living organisms as well as in pharmacology. The main goal of this paper is to attempt a classification of all known enzymes with asparaginase activi...

journal_title：Acta biochimica Polonica

authors： Borek D,Jaskólski M

更新日期：2001-01-01 00:00:00

abstract：:One of the most unusual features of RNA viruses is their enormous genetic variability. Among the different processes contributing to the continuous generation of new viral variants RNA recombination is of special importance. This process has been observed for human, animal, plant and bacterial viruses. The collected d...

journal_title：Acta biochimica Polonica

authors： Alejska M,Kurzyńska-Kokorniak A,Broda M,Kierzek R,Figlerowicz M

更新日期：2001-01-01 00:00:00

abstract：:Potassium permanganate oxidation of pyrimidine bases is often used to probe single-stranded regions in functional DNA-protein complexes. However, so far reactivity of these bases in double-stranded DNA has not been studied quantitatively. We have investigated the kinetics of oxidation of pyrimidines in supercoiled pDS...

journal_title：Acta biochimica Polonica

authors： Loziński T,Wierzchowski KL

更新日期：2001-01-01 00:00:00

abstract：:This review describes the recently developed GeneChip technology that provides efficient access to genetic information using miniaturised, high-density arrays of DNA or oligonucleotide probes. Such microarrays are powerful tools to study the molecular basis of interactions on a scale that would be impossible using con...

journal_title：Acta biochimica Polonica

authors： Gabig M,Wegrzyn G

更新日期：2001-01-01 00:00:00